Application examples of glucose phosphate isomerase_Industrial additives

Background and overview^[1]

Phosphoglucose isomerase is a multifunctional protein that is elevated in rheumatoid arthritis. At the same time, cardiac infarction, hepatitis, cirrhosis, etc. can also increase significantly.

Apply^[1-2]

Application 1,

CN201711047916.2 provides a chassis system and application for ATP regeneration. The chassis system for ATP regeneration includes the following 5 enzymes: α-glucan phosphorylase, phosphoglucomutase, Phosphoglucose isomerase, phosphotransketolase and acetate kinase, in addition to the following 7 enzymes: transaldolase, transketolase, ribose-5-phosphate isomerase, ribulose phosphate 3-epimerase isomerase, triose phosphate isomerase, fructose-bisphosphate aldolase and fructose-1,6-bisphosphatase; the chassis system can be coupled with enzyme-catalyzed reactions that require ATP, using starch or maltodextrin for Energy, no coenzyme added, one-pot reaction to regenerate ATP with high efficiency and low cost. It is an economical, efficient, stable and sustainable ATP regeneration system.

Application 2,

CN201910318184.9 discloses a method for bioconverting lignocellulosic biomass to synthesize fructose-1,6-bisphosphate, which belongs to the field of bioengineering technology. The invention discloses a process for synthesizing high value-added fructose 1,6-bisphosphate using lignocellulosic biomass. A double hydrogen bond deep eutectic solvent is used as a pretreatment reagent to remove hemicellulose and lignin in biomass, improve cellulose accessibility, and add cellulose endo- and exo-enzymes to convert cellulose into cellobiose. , add cellobiose phosphorylase, glucose phosphomutase, phosphoglucose isomerase and pyrophosphate-dependent phosphofructokinase to synthesize fructose 1,6-bisphosphate. This invention uses cheap and easily available lignocellulosic biomass as starting material to synthesize high-energy phosphate compounds through biological transformation. It has the characteristics of low cost, mild reaction, simple operation, etc., and helps alleviate environmental pollution caused by traditional straw treatment methods. problem and has very good economic and social benefits.