Applications of Choline Phosphocholine 1 Antibodies_Industrial Additives

Background^[1-3]

Choline phosphocholine 1 antibody is a type of polyclonal antibody that can specifically bind to choline phosphocholine 1. It is mainly used Immunological experiments for detecting choline phosphocholine 1 protein in vitro.

Phosphocholine has a slight fish smell. A deliquescent powdery product. White crystal or crystalline powder. Very soluble in water. Almost insoluble in benzene, chloroform and ether. Soluble in methanol, slightly soluble in ethanol and acetone.

Choline phosphate has a significant regenerative effect on the phospholipid and protein structure of rat liver cell microsomes damaged by carbon tetrachloride poisoning, and the effect is better than that of protoporphyrin. Clinical studies have shown that choline phosphate participates in the synthesis of phospholipids, and has the effects of protecting and strengthening the liver, promoting lipid metabolism and anti-fatty liver; it can also accelerate methyl transfer, supply active methyl groups, and promote liver cell regeneration; in addition, choline phosphate It can synthesize acetylcholine in the body and activate the autonomic nervous system; it can decompose histamine, enhance the activity of renal histamine enzyme, and have detoxification effect.

Choline phosphocholine 1 antibody structure diagram

Application^[4][5]

Research on soybean transformation with choline phosphotransferase gene

Soybean is an important economic crop, and Heilongjiang Province is It is the main soybean-producing area in my country, but due to low temperature and cold damage, it often causes a substantial reduction in production.

Introduce the choline phosphotransferase gene (cpt) of Brassica napus into soybeans, and solve the problem of cold resistance of soybeans through the direct introduction of foreign gene photoinitiator technology. Plant cold resistance induced by low temperature is related to lipid metabolism. Choline phosphotransferase (CPT) is an integrated membrane protein and an important enzyme in the phospholipid synthesis and metabolism pathway. The product of this enzyme is phosphatidylcholine (PC). , is the main component of biofilm and is closely related to the fluidity and cold resistance of the membrane.

The plasmid pRDH401 containing the 35s-35s-AMV-cpt-NOST fragment was introduced into five common cultivated soybean varieties in Heilongjiang Province through the pollen tube channel method. The obtained seeds were planted and tested in the second year. . After antibiotic and low-temperature screening, PCR detection was performed on potentially transformed plants, and the target gene Poloxamer 407 fragment was amplified from the transformed plants. Southern hybridization was performed on the total DNA of the PCR-positive strains, and the results showed that the foreign genes had been integrated into the genomes of the two soybean varieties. </p