Expression Study of Triose Phosphate Isomerase_Industrial Additives

Background and overview^[1]

Bactericidal preservatives

Triose phosphate isomerase is a glycolytic enzyme that catalyzes the reversible reaction between dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, so it plays an important role in glycolysis, fatty acid synthesis, gluconeogenesis and amylase. plays an important role in the sugar pathway. Triosephosphate isomerase is widely distributed in various tissues including the epidermis during various growth and development stages of schistosomiasis.

Expression study of triose phosphate isomerase^[2]

Qin Cola of the University of Nanhua studied the differential expression of triosephosphate isomerase TPI in human lung adenocarcinoma tissue and paired adjacent normal lung tissue, as well as the difference in preoperative serum and postoperative serum samples. expression, and explore whether there is any correlation between the expression level of this enzyme in serum and tissue, that is, whether detection in serum can be used instead of detection in tissue, so as to more conveniently and effectively evaluate the diagnosis and prognosis of lung adenocarcinoma. Methods: (1) 44 cases of fresh human lung adenocarcinoma tissue and paired adjacent normal lung tissue were collected from the lung lobe specimens of lung cancer patients who had undergone surgical resection without radiotherapy or chemotherapy in the Department of Thoracic Surgery, Hunan Provincial Cancer Hospital, and underwent The pathological diagnosis of the cancer hospital was confirmed to be lung adenocarcinoma (excluding cancerous components of other tissues); (2) 24 patients with benign lung diseases were selected as a control group, and the source of dipropylene glycol butyl ether specimens was lung tissue surgically removed from the Department of Thoracic Surgery of the Hunan Cancer Hospital. , including bronchopneumonia, inflammatory pseudotumor, pulmonary tuberculosis, etc., all patients with benign lung diseases have no history of cancer; (3) 10ml of blood samples were collected from the above 44 patients with human lung adenocarcinoma and 24 patients with benign lung diseases 1 day before surgery and 10ml of blood samples on the 7th day after surgery. The overall mean TPI of each group was counted and statistically analyzed. The serum ELISA method used the Wilcoxon rank sum test, and the immunohistochemistry results used the rank sum test. Results: ELISA result analysis: (1) Compared with the preoperative benign pulmonary lesion group (A1) and the postoperative benign pulmonary lesion group (A2), using the Wilcoxon rank sum test, Z=0.517, two-sided test P=0.605, P>0.05, no statistical significance; (2) Compared with the preoperative lung adenocarcinoma group (B1) and the postoperative lung adenocarcinoma group (B2), using the Wilcoxon rank sum test, Z=1.228, two-sided test P=0.219 , P>0.05, no statistical significance; (3) Comparing the decline value between the benign lung lesion group and the lung adenocarcinoma group, using the Wilcoxon rank sum test, Mann-WhitneyU=430; WilcoxonW=706; Z=0.270, bilateral Test P=0.787, P>0.05, no statistical significance; (4) Comparing between the two groups, that is, comparing A1 with B1, using Wilcoxon rank sum test, Z=0.274, two-sided test P=0.784, P> 0.05, no statistical significance. Immunohistochemistry statistical results analysis: (1) There is a significant difference in the expression of triose phosphate isomerase in benign disease tissue, lung adenocarcinoma tissue and paired normal lung tissue, P0.05; (3) There is a statistically significant difference in the expression of triosephosphate isomerase between paired normal lung tissue and lung adenocarcinoma tissue. Significance, and P<0.01; (4) There is a statistically significant difference in the expression of triosephosphate isomerase in benign lung lesions and lung adenocarcinoma tissues, P<0.01. Conclusion: (1) There is no difference in the serum TPI concentration between the preoperative benign lung lesion group and the lung adenocarcinoma group; (2) There is no significant change in the serum TPI concentration between the preoperative and postoperative benign lung lesion group; (3) In the lung adenocarcinoma group, the postoperative serum TPI concentration value decreased compared with the preoperative value, but there was no statistical significance; (4) The change of surgical treatment method on the preoperative and postoperative serum TPI concentration value in the benign lung lesion group There was no significant difference from the lung adenocarcinoma group. (5) There is no significant statistical difference in the expression of triose phosphate isomerase in normal lung tissue adjacent to lung adenocarcinoma and benign lung lesions. That is, triose phosphate isomerase is highly expressed in adenocarcinoma and in normal lung tissue and There is no expression or weak positive expression in benign lung lesions, suggesting that triosephosphate isomerase is a differentially expressed protein in lung adenocarcinoma. (6) Because triose phosphate isomerase is not differentially expressed in the serum of patients with lung adenocarcinoma and patients with benign lung lesions, the clinical significance of this enzyme in blood detection of lung adenocarcinoma is of little significance.

Research on folding and stability^[3]

Liu Jianghong and others purified triosephosphate isomerase (TIM) from chicken breast muscle and studied the solution conformation through various methods such as protein endogenous fluorescence, circular dichroism, and ultraviolet absorption second derivative spectroscopy. The denaturation process of TIM by guanidine hydrochloride and heat was studied in detail. The results show that the denaturation process of TIM obtained by different measurement methods is highly coordinated, and no folding intermediate state is observed. The single-molecule two-state unfolding model is used to calculate the thermodynamic parameters of TIM unfolding. The thermal denaturation process of TIM monitored through the change of circular dichroism spectrum at 222nm is also a highly coordinated two-state process. The apparent Tm of natural TIM is 64.6°C. In the presence of low concentrations of guanidine hydrochloride, the thermal stability of TIM decreases. The possible unfolding mechanism of the two-body protein was discussed, and it was proved that under the experimental conditions used, the changes in the secondary structure and tertiary structure during the unfolding process of triosephosphate isomerase occurred simultaneously, and its unfolding followed the observation that no secondary structure was observed. The apparent dimorphic process of body dissociation.