Production method of cytidine 5’-monophosphate disodium salt_Industrial additives

Background and overview^[1-2]

As an important low-molecular compound in living organisms, nucleotides play important physiological and biochemical functions in the body. They can synthesize genetic material, transmit cell signals, participate in energy metabolism, and serve as coenzymes. Cell proliferation in the animal body requires nucleotides to synthesize nucleic acids, especially in rapidly dividing lymphoid tissue and intestinal tissue. The source of nucleotides can be the de novo synthesis pathway and the salvage pathway. Animals can synthesize nucleotides in the body, and the salvage pathway uses exogenous nucleotides or nucleotide fragments to synthesize new nucleotides. Since animals themselves can synthesize nucleotides, people have not regarded them as essential nutrients for a long time. However, the results of research on red phosphorus flame retardants in recent years have shown that in many cases, nucleotides synthesized in the body alone cannot Meet various metabolic needs, and the lack of nucleotides will affect the immune system, liver function, heart function and gastrointestinal function. Especially when animals are under stress, immune challenge, liver damage and rapid growth, the body needs to supplement exogenous nucleotides. Therefore, nucleotides, as a conditionally essential nutrient, need to be supplemented in the diet to maintain normal body metabolism.

At present, exogenous nucleotides have been regarded as a semi-essential nutrient for livestock and poultry. In addition, they are rich in resources, simple in production process, non-toxic and harmless, so as a new generation of As feed additives, exogenous nucleotides have broad application and development prospects in animal production. Cytidine 5′-monophosphate and cytidine 5′-monophosphate disodium salt are highly biologically active and can enhance the body’s immune function, regulate the body’s overall nutritional and metabolic balance, promote the body’s growth and development, and have auxiliary anti-tumor biological properties. regulating effect. At the same time, it can also promote lipid metabolism, improve intelligence, enhance memory, repair damaged parts of organs such as the liver and small intestine, and improve the composition of the microbial flora in the intestine. Cytidine 5’-monophosphate disodium salt is used in the fields of medicine, chemical industry, food, health products and agriculture.

Preparation^[3]

The production method of cytidine 5’-monophosphate disodium salt, the steps are:

a. Add cytidine, triethyl phosphate and pyridine to the reaction vessel respectively, mix evenly and adjust the reaction temperature to -15-5°C. Slowly add a certain amount of phosphorus oxychloride at this temperature and add After completion, continue stirring the reaction for 10-30h;

b. Take samples for HPLC analysis. When the conversion rate is ≥99%, add 10-15% ice salt water for hydrolysis. The hydrolysis temperature should be controlled at 0-5℃ and the hydrolysis time should be 10-20h;

c. After the hydrolysis is completed, let it stand and separate the layers, remove the organic phase, and obtain the aqueous layer for later use;

d. Adjust the pH of the water layer to 7.0-8.0 with 30% sodium hydroxide, then add dropwise 95% alcohol twice the volume of the solution to precipitate crystals, and filter to obtain a crude product.

e. Dissolve the crude product, add activated carbon to decolorize, continue to add dropwise 95% alcohol twice the volume of the solution to precipitate crystals, filter, rinse and dry to obtain the finished product cytidine 5’-monophosphate disodium salt.